Anticoagulant Activity of Crude and Phenolic Extracts of Dalbergia ecastaphyllum (L.) Taub. Dried Leaves

Pharmacognosy Research,2021,13,3,121-128.DOI:10.5530/pres.13.3.3Published:June 2021Type:Original ArticleAuthors:Enrico Rogatto Rovetta, Mara Angelina Galvão Magenta, Thaís Moura Gascon, Fernando Luiz Affonso Fonseca, Robson Miranda da Gama, José Armando Jr, and Horácio Dorigan Moya Author(s) affiliations:Enrico Rovetta1, Magenta2, Gascon3, Fonseca3, Gama1, Jr1, Moya1,* 1Centro Universitário FMABC, Pharmacy Laboratory, Santo André, São Paulo, BRAZIL. 2Universidade Santa Cecília, Santos, 3Centro Clinical Analysis 4Centro Analytical Chemistry Abstract:Background: Ischemic diseases are one of the leading causes death worldwide; therefore, search for new compounds with safer more effective anticoagulant antiplatelet action becomes relevant. Objectives: To evaluate impact Dalbergia ecastaphyllum (L.) Taub. dry leaf extract, a species Fabaceae family that is commonly found in Brazil, on blood coagulation. Materials Methods: A statistical design experiments was carried out selection five factors two levels, resulting 32 different ethanolic raw extracts. The extracts were resuspended saline tested pool human blood. activated partially thromboplastin time (APTT) prothrombin (PT) analysed Thrombolyzer®. Results: Treatment using ethanol 70%, drug/solvent ratio 5%, pH 9 dynamic maceration 6 hrs resulted best combination extraction total phenols, while 5% 24 showed flavonoid extraction, being inconclusive. On average, all capable increasing both APTT PT, especially those lower dilutions (1:5); however, overall efficacy Conclusion: under study important results clotting process vitro, mean which suggests has some potential as possible drug class. Keywords:Anticoagulant activity, Taub., Flavonoids, Phenolic compounds, Plant extractsView:PDF (903.36 KB)